Selected recent publications from the Broderick lab:

Pagnier, A., Yang, H., Jodts, R. J., James, C. D., Shepard, E. M., Impano, S., Broderick, W. E., Hoffman, B. M., and Broderick, J. B. (2020) Radical SAM Enzyme Spore Photoproduct Lyase: Properties of the Omega Organometallic Intermediate and Identification of Stable Protein Radicals Formed during Substrate-Free Turnover. J Am Chem Soc 142, 18652-18660

Nemeth, B., Senger, M., Redman, H. J., Ceccaldi, P., Broderick, J., Magnuson, A., Stripp, S. T., Haumann, M., and Berggren, G. (2020) [FeFe]-hydrogenase maturation: H-cluster assembly intermediates tracked by electron paramagnetic resonance, infrared, and X-ray absorption spectroscopy. J Biol Inorg Chem 25, 777-788

Yang, H., Impano, S., Shepard, E. M., James, C. D., Broderick, W. E., Broderick, J. B., and Hoffman, B. M. (2019) Photoinduced Electron Transfer in a Radical SAM Enzyme Generates an S-Adenosylmethionine Derived Methyl Radical. J Am Chem Soc 141, 16117-16124

Byer, A. S., Shepard, E. M., Ratzloff, M. W., Betz, J. N., King, P. W., Broderick, W. E., and Broderick, J. B. (2019) H-cluster assembly intermediates built on HydF by the radical SAM enzymes HydE and HydG. J Biol Inorg Chem 24, 783-792

Galambas, A., Miller, J., Jones, M., McDaniel, E., Lukes, M., Watts, H., Copie, V., Broderick, J. B., Szilagyi, R. K., and Shepard, E. M. (2019) Radical S-adenosylmethionine maquette chemistry: Cx3Cx2C peptide coordinated redox active [4Fe-4S] clusters. J Biol Inorg Chem 24, 793-807

Broderick, W. E., and Broderick, J. B. (2019) Radical SAM enzymes: surprises along the path to understanding mechanism. J Biol Inorg Chem 24, 769-776

Yang, H., McDaniel, E. C., Impano, S., Byer, A. S., Jodts, R. J., Yokoyama, K., Broderick, W. E., Broderick, J. B., and Hoffman, B. M. (2019) The Elusive 5′-Deoxyadenosyl Radical: Captured and Characterized by Electron Paramagnetic Resonance and Electron Nuclear Double Resonance Spectroscopies. J Am Chem Soc 141, 12139-12146

Hanscam, R., Shepard, E. M., Broderick, J. B., Copie, V., and Szilagyi, R. K. (2019) Secondary structure analysis of peptides with relevance to iron-sulfur cluster nesting. J Comput Chem 40, 515-526

Adelson, C. N., Johnston, E. M., Hilmer, K. M., Watts, H., Dey, S. G., Brown, D. E., Broderick, J. B., Shepard, E. M., Dooley, D. M., and Solomon, E. I. (2019) Characterization of the Preprocessed Copper Site Equilibrium in Amine Oxidase and Assignment of the Reactive Copper Site in Topaquinone Biogenesis. J Am Chem Soc 141, 8877-8890

William E. Broderick, Brian M. Hoffman, Joab B Broderick, “Mechanism of Radical Initiation in the Radical S-Adenosyl-L-methionine Superfamily,” Acc. Chem. Res. 2018, 51(11), 2611–2619.

Amanda S. Byer, Hao Yang, Elizabeth C. McDaniel, Venkatesan Kathiresan, Stella Impano, Adrien Pagnier, Hope Watts, Carly Denler, Anna L. Vagstad, Jorn Piel, Kaitlin S. Duschene, Eric M. Shepard, Thomas P. Shields, Lincoln G. Scott, Edward A. Lilla, Kenichi Yokoyama, William E. Broderick, Brian M. Hoffman, and Joan B. Broderick, “Paradigm shift for radical S-adenosyl-l-methionine reactions: the organometallic intermediate Ω is central to catalysis,” J. Am. Chem. Soc. 2018, 140, 8634-8638. 10.1021/jacs.8b04061

Anna G. Scott, Robert K. Szilagyi, David W. Mulder, Michael W. Ratzloff, Amanda S. Byer, Paul W. King, William E. Broderick, Eric M. Shepard, and Joan B. Broderick, “Compositional and structural insights into the nature of the H-cluster precursor on HydF,” Dalton Trans. 2018, 47, 9521-9535. 10.1039/c8dt01654b

Eric M. Shepard, Amanda S. Byer, and Joan B. Broderick, “Iron-Sulfur Cluster States of the Hydrogenase Maturase HydF,” Biochemistry 2017, 56, 4733-4734.  10.1021/acs.biochem.7b00735

Krista A. Shisler, Rachel U. Hutcheson, Masaki Horitani, Kaitlin S. Duschene, Adam V. Crain, Amanda S. Byer, Eric M. Shepard, Ashley Rasmussen, Jian Yang, William E. Broderick, Jessica L. Vey, Catherine L. Drennan, Brian M. Hoffman, and Joan B. Broderick, “Monovalent cation activation of the radical SAM enzyme pyruvate formate-lyase activating enzyme,” J. Am. Chem. Soc. 2017, 139, 11803-11813.  10.1021/jacs.7b04883


Eric M. Shepard, Amanda S. Byer, Priyanka Aggarwal, Jeremiah N. Betz, Anna G. Scott, Krista A. Shisler, Robert J. Usselman, Gareth R. Eaton, Sandra S. Eaton, and Joan B. Broderick, “Electron spin relaxation and biochemical characterization of the hydrogenase maturase HydF: Insights into [2Fe-2S] and [4Fe-4S] cluster communication and hydrogenase activation,” Biochemistry 2017, 56, 3234-3247.  10.1021/acs.biochem.7b00169

Eric M. Shepard, Amanda S. Byer, Jeremiah N. Betz, John W. Peters, and Joan B. Broderick, “A Redox Active [2Fe-2S] Cluster on the Hydrogenase Maturase HydF,” Biochemistry 2016, 55(25), 3514-3527.  10.1021/acs.biochem.6b00528

Masaki Horitani, Krista Shisler, William E. Broderick, Rachel U. Hutcheson, Kaitlin S. Duschene, Amy R. Marts, Brian M. Hoffman, and Joan B. Broderick, “Radical SAM catalysis via an organometallic intermediate with an Fe-[5’-C]-deoxyadenosyl bond,” Science 2016, 352(6287), 822-825.  10.1126/science.aaf5327

Masaki Horitani, Amanda S. Byer, Krista A. Shisler, Tilak Chandra, Joan B. Broderick, Brian M. Hoffman, “Why Nature uses radical SAM enzymes so widely: ENDOR studies of lysine 2,3-aminomutase shows the 5’-dAdo• ‘Free Radical’ is never free,” J. Am. Chem. Soc. 2015, 137(22) , 7111 – 7121.  10.1021/jacs.5b00498